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Peptide testing glossary Plain-language peptide testing terms with practical examples: purity, content, reporting limits, microbial units, calibration and batch traceability.
Updated September 12, 2026 3 min read
Sample and document terms Term Meaning and example Batch / lot A defined group linked to records. The full lot connects supplied material to its test record; a product name alone does not. Laboratory sample ID The lab’s own identifier. It may differ from the vial lot, so a recorded connection is needed. COA Certificate of Analysis: findings for an identified submitted sample, not a universal certificate for a brand. Specification The requirement used to assess a result. “NLT 98%” is a threshold, not a measured 98% result. Revision A changed version of a report. Use the current issued version and retain the record of the change.
Composition and amount Term Meaning and example Purity Relative composition on the stated analytical basis. Peak-area purity is not automatically a mass percentage. Identity Evidence that material matches the intended compound. High purity alone cannot prove the right compound. Quantity Measured amount, such as mg/vial. A 9 mg result is not changed by a separate 99% purity figure. Peptide content A method-defined content value. Counterions and moisture can make gross powder mass differ from peptide mass. Concentration Amount per volume, such as mg/mL. It needs the relevant preparation volume. Blend ratio The relative amounts of components. A total alone cannot establish each amount.
Methods and signals Term Meaning and example Chromatogram Detector signal over separation time. One visible peak can include overlapping components. HPLC High-performance liquid chromatography; separates components under specified conditions. LC-MS Liquid chromatography plus mass spectrometry; adds mass-to-charge evidence. m/z Mass-to-charge ratio. Multiple charges mean a peak need not equal the neutral molecular mass. ICP-MS Elemental analysis for named targets; not a peptide identity test. Calibration Relating instrument response to references so a measurement can be interpreted quantitatively.
Limits and result wording Term Meaning and example LOD Limit of detection: the procedure’s detection capability under defined conditions. LOQ Limit of quantitation: the lower boundary for adequately reliable numerical measurement under the method. ND Not detected. It does not mean absolute zero or cover unnamed targets. NMT / NLT Not more than / not less than the stated requirement. Pending A final finding is not yet available; it is not a pass. Uncertainty Evaluated dispersion associated with a measurement. It is not permission to pick the most favorable value. Accuracy / precision Agreement with a reference / consistency of repeated measurements. These differ.
Microbiology and records Term Meaning and example EU Endotoxin units: activity, not the number of bacteria. CFU Colony-forming units: recovered growth, not necessarily individual cells. Bioburden Microbial burden assessed by a defined method. Low counts do not establish sterility. Method suitability Evidence that the procedure can work in the sample’s presence, including relevant interference or recovery checks. Batch traceability The vial-to-batch-to-report record connection. Measurement traceability A documented calibration connection to a reference; different from a lot-number match.
Read this example in full “Endotoxin <0.1 EU/mL” describes activity below a reporting boundary in a stated preparation. It does not mean “zero bacteria,” “less than 0.1 mg,” or “every vial sterile.” This is an illustrative value, not a Morris Peppy result.
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